استفاده از روش سطح پاسخ برای مدل‌سازی و بهینه‌سازی برداشت ریزجلبکChlorella sorokiniana pa.91 با Fe3O4/PAClازمحیط کشت فاضلاب شهری

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه مهندسی عمران محیط ‌‌زیست، دانشکده مهندسی عمران، دانشگاه صنعتی نوشیروانی بابل، بابل، ایران

چکیده

ریزجلبک‌ها به عنوان یک منبع پایدار برای تولید محصولات زیستی با ارزش مورد توجه هستند و کاربردهایی در صنایعی مانند افزودنی‌های غذایی، ترکیبات زیست فعال، داروسازی و آرایشی پیدا می‌کنند. با این حال، یکی از چالش‌های اصلی در استفاده از ریزجلبک‌ها برای این اهداف، برداشت کارآمد زیست‌توده می‌باشد که به عنوان یک گلوگاه اصلی در پردازش پایین دست عمل می‎کند. یکی از مکانیزم‌های جایگزین برای برداشت ریزجلبک از محیط کشت استفاده از روش منعقدکننده مغناطیسی است. در این مطالعه، فرآیند برداشت ریزجلبک بومی  Chlorella sorokiniana pa.91را با استفاده از Fe3O4/PACl در فرآیند لخته‌سازی مورد بررسی قرار داده شده است. در این تحقیق از نانوذرات  Fe3O4سنتز شده به روش هم‌رسوبی برای برداشت ریزجلبک‌ها استفاده شد. با استفاده روش سطح پاسخ، برای بررسی راندمان حذف، بهینه‌سازی مهم‌ترین پارامترهای فرآیند برداشت انعقاد مغناطیسی شامل زمان، غلظت نانومواد و pH در محیط کشت حاصل از فاضلاب شهری بررسی شده است. تجزیه و تحلیل تجربی نشان داد که بالاترین راندمان برداشت تقریبا90 درصد، در شرایط 3/5 گرم در لیتر  Fe3O4 غلظت ثابت  0/075 ‌گرم در لیتر PACl ، زمان برداشت 40 دقیقه وpH  4 به دست آمده است از طرفی کمترین مقدار راندمان برداشت ریزجلبک در شرایطی که مقدار نانو ذره کامپوزیتی برابر با 0/5 گرم در لیتر، مدت زمان برداشت 27/5 دقیقه و  pH6/5 برابر با 22 درصد گزارش شده است. در این میان، بازی‌ترین حالت از pH که برابر با 11.5 است راندمان حذف ریزجلبک 53% و در اسیدی‌ترین حالت برابر 75% می‌باشد و همانطور میزان بیشترین راندمان برداشت در pH خنثی با مدت زمان ته‌نشینی 53 دقیقه برابر با 80 % مشاهده شده است. مقدار ضریب تعیین برای این طرح (R2)  0/9823 به دست آمده، نشان‌دهنده رابطه قوی بین متغیرهای مستقل و مقادیر پاسخ است. علاوه بر این، حالت ترکیبی مورد مطالعه قرار گرفته راندمان لخته‌سازی ریزجلبک‌ها را بهبود می‌بخشد. از نتایج به دست آمده می‌توان استنباط کرد با افزایش زمان و غلظت نانو ذرات و کاهش pH راندمان برداشت ریزجلبک افزایشی خواهد بود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Use of response surface method for modeling and optimization of harvesting of Chlorella sorokiniana pa.91 with Fe3O4/PACl from municipal wastewater

نویسندگان [English]

  • Sara Akbari
  • Farhad Qaderi
  • hasan amini rad
Babol Noshirvani University of Technology
چکیده [English]

Microalgae have the potential to produce valuable substances for pharmaceutical purposes as well as serve as a food source, providing bioactive compounds and ingredients for cosmetics. However, harvesting microalgae is a crucial step in the mass production of various high-value products derived from microalgae. This process often becomes a major bottleneck in downstream processing. It is essential to find effective and cost-effective harvesting methods for industrial applications. Among several harvesting methods, magnetic flocculation offers the benefits of modest operation, energy savings, and quick separation. This study investigates the harvesting process of Chlorella sorokiniana pa.91 microalgae using a novel flocculation process involving nano-Fe3O4 coated with PACl. In this research, we have used the chemical co-precipitation method to prepare nanoparticles. Using the response surface method to optimize the most important parameters of the magnetic flocculation harvesting process to check the microalgae removal efficiency, three variables of time, concentration of nanomaterials, and pH in the culture medium obtained from municipal wastewater have been investigated. The results demonstrated that the highest harvesting efficiency, nearly 90%, was achieved under the conditions of 3.5 g/L Fe3O4, a constant concentration of 0.075 g/L PACl, a harvesting duration of 40 minutes, and a pH level of 4. On the other hand, the lowest microalgae harvesting efficiency was observed under specific conditions: a composite nanoparticle concentration of 0.5 g/L per liter, a harvesting time of 27.5 minutes, and a pH of 6.5 resulting in a mere 22% efficiency. The rate of microalgal removal increased from 53% in the most alkaline condition to 75% in the most acidic environment. The highest harvesting efficiency, reaching 80%, was achieved under neutral pH conditions with a settling time of 53 minutes. Furthermore, the investigated combined method investigated enhances the flocculation effectiveness of microalgae. Based on the findings, it can be inferred that the efficiency of microalgae harvesting rises with longer duration, higher nanoparticle concentrations, and lower pH levels

کلیدواژه‌ها [English]

  • Harvesting
  • microalgae
  • municipal wastewater
  • surface response method
  • Fe3O4/PACl composite nanoparticles

مراجع

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نشریه مهندسی عمران امیرکبیر
دوره 56، شماره 2
1403
صفحه 181-202

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