تصفیه فاضلاب شهری به وسیله ریزجلبک بومی Chlorella.sorokiniana pa.19 با استفاده از نانو ماده آمینوکلی‌منیزیم

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشکده عمران و محیط‌زیست، دانشگاه صنعتی نوشیروانی بابل، بابل، ایران.

چکیده

ریزجلبک‌ها به‌عنوان میکروارگانیزم‌های آبی تک‌سلولی یوکاریوتی می‌توانند برای تصفیه انواع مختلف فاضلاب شهری و صنعتی مورد استفاده قرار گیرند. امروزه به دلیل هزینه بالای سنتز محیط کشت در مقیاس صنعتی، می‌توان از فاضلاب به‌عنوان محیط کشت مناسب، مغذی، ارزان و قابل‌دسترس استفاده نمود. مهندسی نانومواد به‌عنوان رشته نسبتاً جدید، راه‌حل‌های مؤثری را برای بهینه‌سازی فرآیند تصفیه فاضلاب به وسیله ریزجلبک پیشنهاد داده است. هدف این پژوهش، بررسی تجربی تأثیر نانوماده آمینوکلی‌منیزیم بر رشد زیست‌توده ریزجلبک بومی Chlorella.sorokiniana pa.19 در محیط کشت فاضلاب خانگی شهر ساری بود. در این راستا، تأثیر غلظت‌های مختلف نانو ماده آمینوکلی‌منیزیم سنتز شده بر وزن خشک زیست‌توده، نرخ رشد مخصوص، حذف آمونیاک و فسفات مورد بررسی قرار گرفت. به منظور بررسی مشخصات و کیفیت نانوماده سنتز شده از آنالیز طیف‌سنجی پراش اشعه ایکس (XRD)، میکروسکوپ الکترونی روبشی (FE-SEM) بر روی نانو ماده و آنالیز میکروسکوپ نیروی اتمی (AFM) بر روی ریزجلبک Chlorella.sorokiniana pa.19 انجام شد. مقادیر وزن خشک زیست‌توده، نرخ رشد مخصوص، بازده حذف آمونیاک و فسفات به ترتیب 67/16، 43/01، 98/33 و 96/87 درصد در دمای 28درجه سلسیوس، شدت تابش 2800 لوکس و مقدار 0/2 گرم در لیتر نانو ماده آمینوکلی‌منیزیم مشاهده شد. در غلظت کم نانوماده آمینوکلی‌منیزیم افزایش کمی و کیفی توده زیستی ریزجلبک مشاهده شد و غلظت‌های بالاتر از دوز بهینه سبب کاهش میزان رشد گونه شد. استفاده هم‌زمان نانوماده و ریزجلبک در تصفیه فاضلاب به عنوان روش دوست‌دار محیط زیست موجب بهبود وضعیت اکولوژیکی منابع آب، به ویژه با کاهش نیتروژن و فسفر در پساب فاضلاب خواهد شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Treatment of Municipal Wastewater by Chlorella. sorokiniana pa.19 Using Magnesium-Aminoclay Nanomaterial

نویسندگان [English]

  • Masoumeh Panbehkar Bisheh
  • hasan amini rad
شهرآرا-خ پاتریس لومومبا-روبروی بانک کشاورزی-ک بلوک بیستم- 6/2
چکیده [English]

Microalgae, as unicellular eukaryotic aquatic microorganisms, can treat various urban and industrial wastewater types. Due to the high cost of synthesis of culture medium on an industrial scale, wastewater is used as a cheap and accessible culture medium. Recent advances in the field of nanoengineering have facilitated the application of nanotechnology in wastewater treatment. As a relatively new field, nanomaterials engineering has proposed effective solutions for most technical problems in microalgae biodegradation. This research aimed to experimentally investigate the effect of amino-clay-magnesium nanomaterial on the growth of native microalgae Chlorella. sorokiniana pa.19 biomass in the sewage culture medium of Sari city. In this regard, the effect of the concentration of magnesium-amino clay nanomaterial on biomass dry weight, specific growth rate, ammonia, and phosphate removal in an urban sewage culture medium was investigated. X-ray diffraction (XRD) analysis, scanning electron microscopy (FE-SEM) on the nanomaterial, and atomic force microscopy (AFM) analysis were performed on the microalgae. Biomass dry weight values, specific growth rate, ammonia, and phosphate removal efficiency are 16.67, 43.01, 98.33, and 96.87%, respectively, at 28 o C, 2800 lux radiation intensity, and 0.2 grams per liter nano magnesium-amino clay material was observed. At a low concentration of magnesium-amino clay nanomaterial, a quantitative and qualitative increase in the biomass of Chlorella. sorokiniana pa.19 microalgae was observed, and at concentrations higher than the optimal dose, it caused a decrease in the growth rate of the species.

کلیدواژه‌ها [English]

  • Microalgae
  • Wastewater
  • Magnesium-Amino clay Nanomaterial
  • XRD
  • FE-SEM
[1] D. Kumar, T. Das, B.S. Giri, B. Verma, Preparation and characterization of novel hybrid bio-support material immobilized from Pseudomonas cepacia lipase and its application to enhance biodiesel production, Renewable Energy, 147 (2020) 11-24.
[2] P. Yaqoubnejad, H.A. Rad, M. Taghavijeloudar, Development a novel hexagonal airlift flat plate photobioreactor for the improvement of microalgae growth that simultaneously enhance CO2 bio-fixation and wastewater treatment, Journal of Environmental Management, 298 (2021) 113482.
[3] S. Maryjoseph, B. Ketheesan, Microalgae based wastewater treatment for the removal of emerging contaminants: A review of challenges and opportunities, Case studies in chemical and environmental engineering, 2 (2020) 100046.
[4] M.Y. Alazaiza, S. He, D. Su, S.S. Abu Amr, P.Y. Toh, M.J. Bashir, Sewage water treatment using Chlorella vulgaris microalgae for simultaneous nutrient separation and biomass production, Separations, 10(4) (2023) 229.
[5] L.B. Sukla, E. Subudhi, D. Pradhan, The role of microalgae in wastewater treatment, Springer, 2019.
[6] H. Al-Jabri, P. Das, S. Khan, M. Thaher, M. AbdulQuadir, Treatment of wastewaters by microalgae and the potential applications of the produced biomass—a review, Water, 13(1) (2020) 27.
[7] S. Kazemifard, H. Nayebzadeh, N. Saghatoleslami, E. Safakish, Application of magnetic alumina-ferric oxide nanocatalyst supported by KOH for in-situ transesterification of microalgae cultivated in wastewater medium, Biomass and Bioenergy, 129 (2019) 105338.
[8] C. Urrutia, E. Yañez-Mansilla, D. Jeison, Bioremoval of heavy metals from metal mine tailings water using microalgae biomass, Algal Research, 43 (2019) 101659.
[9] H. Eladel, A.E.-F. Abomohra, M. Battah, S. Mohmmed, A. Radwan, H. Abdelrahim, Evaluation of Chlorella sorokiniana isolated from local municipal wastewater for dual application in nutrient removal and biodiesel production, Bioprocess and biosystems engineering, 42 (2019) 425-433.
[10] B. Gatamaneni Loganathan, V. Orsat, M. Lefsrud, Phycoremediation and valorization of synthetic dairy wastewater using microalgal consortia of Chlorella variabilis and Scenedesmus obliquus, Environmental Technology, 42(20) (2021) 3231-3244.
[11] D. Zhou, C. Zhang, L. Fu, L. Xu, X. Cui, Q. Li, J.C. Crittenden, Responses of the microalga Chlorophyta sp. to bacterial quorum sensing molecules (N-acylhomoserine lactones): aromatic protein-induced self-aggregation, Environmental Science & Technology, 51(6) (2017) 3490-3498.
[12] P. Asadi, H.A. Rad, F. Qaderi, Comparison of Chlorella vulgaris and Chlorella sorokiniana pa. 91 in post treatment of dairy wastewater treatment plant effluents, Environmental Science and Pollution Research, 26 (2019) 29473-29489.
[13] R.G. Saratale, S.-K. Cho, R.N. Bharagava, A.K. Patel, S. Varjani, S.I. Mulla, D.S. Kim, S.K. Bhatia, L.F.R. Ferreira, H.S. Shin, A critical review on biomass-based sustainable biorefineries using nanobiocatalysts: Opportunities, challenges, and future perspectives, Bioresource Technology, 363 (2022) 127926.
[14] S. Vasistha, A. Khanra, M. Clifford, M.P. Rai, Current advances in microalgae harvesting and lipid extraction processes for improved biodiesel production: A review, Renewable and Sustainable Energy Reviews, 137 (2021) 110498.
[15] M.K. Nguyen, J.-Y. Moon, Y.-C. Lee, Loading effects of low doses of magnesium aminoclay on microalgal Microcystis sp. KW growth, macromolecule productions, and cell harvesting, Biomass and Bioenergy, 139 (2020) 105619.
[16] J.-Q. Xiong, S. Ru, Q. Zhang, M. Jang, M.B. Kurade, S.-H. Kim, B.-H. Jeon, Insights into the effect of cerium oxide nanoparticle on microalgal degradation of sulfonamides, Bioresource technology, 309 (2020) 123452.
[17] S. Vasistha, A. Khanra, M.P. Rai, Influence of microalgae-ZnO nanoparticle association on sewage wastewater towards efficient nutrient removal and improved biodiesel application: An integrated approach, Journal of Water Process Engineering, 39 (2021) 101711.
[18] P. Asadi, H.A. Rad, F. Qaderi, Lipid and biodiesel production by cultivation isolated strain Chlorella sorokiniana pa. 91 and Chlorella vulgaris in dairy wastewater treatment plant effluents, Journal of Environmental Health Science and Engineering, 18 (2020) 573-585.
[19] J. Baird, L. Schultz, R. Plummer, D. Armitage, Ö. Bodin, Emergence of collaborative environmental governance: what are the causal mechanisms?, Environmental management, 63 (2019) 16-31.
[20] S. Nayak, I. Khozin-Goldberg, G. Cohen, D. Zilberg, Dietary supplementation with ω6 LC-PUFA-rich algae modulates zebrafish immune function and improves resistance to streptococcal infection, Frontiers in immunology, 9 (2018) 1960.
[21] M. Nayak, N. Rashid, W.I. Suh, B. Lee, Y.K. Chang, Performance evaluation of different cationic flocculants through pH modulation for efficient harvesting of Chlorella sp. HS2 and their impact on water reusability, Renewable Energy, 136 (2019) 819-827.
[22] W. Farooq, H.U. Lee, Y.S. Huh, Y.-C. Lee, Chlorella vulgaris cultivation with an additive of magnesium-aminoclay, Algal Research, 17 (2016) 211-216.
[23] S. Kim, J.-e. Park, Y.-B. Cho, S.-J. Hwang, Growth rate, organic carbon and nutrient removal rates of Chlorella sorokiniana in autotrophic, heterotrophic and mixotrophic conditions, Bioresource technology, 144 (2013) 8-13.
[24] X. Li, F. Cai, T. Luan, L. Lin, B. Chen, Pyrene metabolites by bacterium enhancing cell division of green alga Selenastrum capricornutum, Science of the Total Environment, 689 (2019) 287-294.
[25] L.N. Nguyen, L. Labeeuw, A.S. Commault, B. Emmerton, P.J. Ralph, M.A.H. Johir, W. Guo, H.H. Ngo, L.D. Nghiem, Validation of a cationic polyacrylamide flocculant for the harvesting fresh and seawater microalgal biomass, Environmental Technology & Innovation, 16 (2019) 100466.
[26] V.K.H. Bui, D. Park, Y.-C. Lee, Aminoclays for biological and environmental applications: An updated review, Chemical Engineering Journal, 336 (2018) 757-772.
[27] L. Fu, K.K.R. Datta, K. Spyrou, G. Qi, A. Sardar, M.M. Khader, R. Zboril, E.P. Giannelis, Phyllosilicate nanoclay-based aqueous nanoparticle sorbent for CO2 capture at ambient conditions, Applied Materials Today, 9 (2017) 451-455.
[28] T.M. Fernandes, B.B. Gomes, U.M. Lanfer-Marquez, Apparent absorption of chlorophyll from spinach in an assay with dogs, Innovative Food Science & Emerging Technologies, 8(3) (2007) 426-432.
[29] H. Begum, F.M. Yusoff, S. Banerjee, H. Khatoon, M. Shariff, Availability and utilization of pigments from microalgae, Critical reviews in food science and nutrition, 56(13) (2016) 2209-2222.
[30] M.M.H. Alejandra Londono-Calderon, Pierre E. Palo, Lee Bendickson,, M.N.-H. Sandra Vergara, Andrew C. Hillier, and Tanya Prozorov, Londono-Calderon et al. - 2019 - Imaging of Unstained DNA Origami Triangles with Electron Microscopy, Small Methods, 1900393 (2019).
[31] B. Kim, R. Praveenkumar, J. Lee, B. Nam, D.-M. Kim, K. Lee, Y.-C. Lee, Y.-K. Oh, Magnesium aminoclay enhances lipid production of mixotrophic Chlorella sp. KR-1 while reducing bacterial populations, Bioresource Technology, 219 (2016) 608-613.
[32] C.M. Hoo, N. Starostin, P. West, M.L. Mecartney, A comparison of atomic force microscopy (AFM) and dynamic light scattering (DLS) methods to characterize nanoparticle size distributions, Journal of Nanoparticle Research, 10 (2008) 89-96.
[33] T.O. Ajiboye, O.A. Oyewo, D.C. Onwudiwe, Simultaneous removal of organics and heavy metals from industrial wastewater: A review, Chemosphere, 262 (2021) 128379.
[34] M.K. Nguyen, J.-Y. Moon, V.K.H. Bui, Y.-K. Oh, Y.-C. Lee, Recent advanced applications of nanomaterials in microalgae biorefinery, Algal Research, 41 (2019) 101522.
[35] P. Spolaore, C. Joannis-Cassan, E. Duran, A. Isambert, Commercial applications of microalgae, Journal of bioscience and bioengineering, 101(2) (2006) 87-96.
[36] A.A. Ansari, A.H. Khoja, A. Nawar, M. Qayyum, E. Ali, Wastewater treatment by local microalgae strains for CO 2 sequestration and biofuel production, Applied Water Science, 7 (2017) 4151-4158.
[37] K. Alemu, B. Assefa, D. Kifle, H. Kloos, Nitrogen and phosphorous removal from municipal wastewater using high rate algae ponds, INAE Letters, 3 (2018) 21-32.
[38] Y. Liu, I. Yildiz, The effect of salinity concentration on algal biomass production and nutrient removal from municipal wastewater by Dunaliella salina, International Journal of Energy Research, 42(9) (2018) 2997-3006.
[39] H. Doshi, A. Ray, I. Kothari, Biosorption of cadmium by live and dead Spirulina: IR spectroscopic, kinetics, and SEM studies, Current Microbiology, 54 (2007) 213-218.